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Plko 1 Puro Shsox2 1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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High levels of MKP1 correlate with GSCs differentiation. a Analysis of the MKP1 mRNA expression levels in GSC lines grown in stem medium compared to differentiation conditions ( n = 3). b MKP1 mRNA expression by RNA-seq analysis in G26 and NS-1 cells at 8, 32, and 64-day time course of BMP treatment after GF withdrawal (GF). Error bars denote SD of four independent experiments. c Frequency of MKP1 promoter methylation after BMP4 continous treatment at the indicated time points. d mRNA expression of MKP1 was analyzed in U87 and GNS166 lines under increasing 5-AZA concentrations. e Analysis of MKP1 mRNA expression levels in U251 cells with knockdown of SOX2 ( <t>shSOX2</t> ) or SOX9 ( shSOX9 ) related to the control ( pLKO ) condition ( n = 3). qRT-PCR data are normalized to GAPDH expression. f MKP1 levels in U87 cells with SOX2 or SOX9 overexpression compared to control cells ( n = 3). g Association analysis of MKP1 with SOX2 and SOX9 in the human glioblastoma TCGA cohort from R2: Genomics Analysis and Visualization Platform http://r2.amc.nl . The analysis showed a negative correlation between MKP1 and SOX2 ( R −0.207, p value 1.3e−06), and MKP1 and SOX9 ( R −0.169, p value 1.7e−04)
Plko 1 Shsox2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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High levels of MKP1 correlate with GSCs differentiation. a Analysis of the MKP1 mRNA expression levels in GSC lines grown in stem medium compared to differentiation conditions ( n = 3). b MKP1 mRNA expression by RNA-seq analysis in G26 and NS-1 cells at 8, 32, and 64-day time course of BMP treatment after GF withdrawal (GF). Error bars denote SD of four independent experiments. c Frequency of MKP1 promoter methylation after BMP4 continous treatment at the indicated time points. d mRNA expression of MKP1 was analyzed in U87 and GNS166 lines under increasing 5-AZA concentrations. e Analysis of MKP1 mRNA expression levels in U251 cells with knockdown of SOX2 ( <t>shSOX2</t> ) or SOX9 ( shSOX9 ) related to the control ( pLKO ) condition ( n = 3). qRT-PCR data are normalized to GAPDH expression. f MKP1 levels in U87 cells with SOX2 or SOX9 overexpression compared to control cells ( n = 3). g Association analysis of MKP1 with SOX2 and SOX9 in the human glioblastoma TCGA cohort from R2: Genomics Analysis and Visualization Platform http://r2.amc.nl . The analysis showed a negative correlation between MKP1 and SOX2 ( R −0.207, p value 1.3e−06), and MKP1 and SOX9 ( R −0.169, p value 1.7e−04)
Plko 1 Puro Shsox2 2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Plko 1 Shsox2 3h B, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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High levels of MKP1 correlate with GSCs differentiation. a Analysis of the MKP1 mRNA expression levels in GSC lines grown in stem medium compared to differentiation conditions ( n = 3). b MKP1 mRNA expression by RNA-seq analysis in G26 and NS-1 cells at 8, 32, and 64-day time course of BMP treatment after GF withdrawal (GF). Error bars denote SD of four independent experiments. c Frequency of MKP1 promoter methylation after BMP4 continous treatment at the indicated time points. d mRNA expression of MKP1 was analyzed in U87 and GNS166 lines under increasing 5-AZA concentrations. e Analysis of MKP1 mRNA expression levels in U251 cells with knockdown of SOX2 ( shSOX2 ) or SOX9 ( shSOX9 ) related to the control ( pLKO ) condition ( n = 3). qRT-PCR data are normalized to GAPDH expression. f MKP1 levels in U87 cells with SOX2 or SOX9 overexpression compared to control cells ( n = 3). g Association analysis of MKP1 with SOX2 and SOX9 in the human glioblastoma TCGA cohort from R2: Genomics Analysis and Visualization Platform http://r2.amc.nl . The analysis showed a negative correlation between MKP1 and SOX2 ( R −0.207, p value 1.3e−06), and MKP1 and SOX9 ( R −0.169, p value 1.7e−04)

Journal: Oncogenesis

Article Title: High expression of MKP1/DUSP1 counteracts glioma stem cell activity and mediates HDAC inhibitor response

doi: 10.1038/s41389-017-0003-9

Figure Lengend Snippet: High levels of MKP1 correlate with GSCs differentiation. a Analysis of the MKP1 mRNA expression levels in GSC lines grown in stem medium compared to differentiation conditions ( n = 3). b MKP1 mRNA expression by RNA-seq analysis in G26 and NS-1 cells at 8, 32, and 64-day time course of BMP treatment after GF withdrawal (GF). Error bars denote SD of four independent experiments. c Frequency of MKP1 promoter methylation after BMP4 continous treatment at the indicated time points. d mRNA expression of MKP1 was analyzed in U87 and GNS166 lines under increasing 5-AZA concentrations. e Analysis of MKP1 mRNA expression levels in U251 cells with knockdown of SOX2 ( shSOX2 ) or SOX9 ( shSOX9 ) related to the control ( pLKO ) condition ( n = 3). qRT-PCR data are normalized to GAPDH expression. f MKP1 levels in U87 cells with SOX2 or SOX9 overexpression compared to control cells ( n = 3). g Association analysis of MKP1 with SOX2 and SOX9 in the human glioblastoma TCGA cohort from R2: Genomics Analysis and Visualization Platform http://r2.amc.nl . The analysis showed a negative correlation between MKP1 and SOX2 ( R −0.207, p value 1.3e−06), and MKP1 and SOX9 ( R −0.169, p value 1.7e−04)

Article Snippet: For SOX2 or SOX9 knockdown, cells were infected with pLKO.1 shSOX2 (a gift from M. Meyerson, Addgene plasmid 26353), shSOX9 (a gift from B. Weinberg, Addgene plasmid 1855), or empty vector.

Techniques: Expressing, RNA Sequencing, Methylation, Knockdown, Control, Quantitative RT-PCR, Over Expression

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Journal: Developmental cell

Article Title: A Yap-Myc-Sox2-p53 Regulatory Network Dictates Metabolic Homeostasis and Differentiation in Kras-driven Pancreatic Ductal Adenocarcinomas

doi: 10.1016/j.devcel.2019.07.022

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Lenti- or retro-viral plasmids pLKO.1 shSox2 HM a (#26353), pLKO.1 shSox2 3H b (#26352), pWZL-Blast-MYC (#10674), and pBABE-YAP (#15682) were purchased from Addgene, Cambridge, MA ( Bass et al., 2009 ; Boehm et al., 2005 ; Overholtzer et al., 2006 ).

Techniques: Recombinant, Viability Assay, Imaging, TUNEL Assay, Methylation, Plasmid Preparation, Polymer, cDNA Synthesis, SYBR Green Assay, Bicinchoninic Acid Protein Assay, DNA Extraction, Amplification, Knock-Out, CRISPR, Software