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Image Search Results
Journal: Oncogenesis
Article Title: High expression of MKP1/DUSP1 counteracts glioma stem cell activity and mediates HDAC inhibitor response
doi: 10.1038/s41389-017-0003-9
Figure Lengend Snippet: High levels of MKP1 correlate with GSCs differentiation. a Analysis of the MKP1 mRNA expression levels in GSC lines grown in stem medium compared to differentiation conditions ( n = 3). b MKP1 mRNA expression by RNA-seq analysis in G26 and NS-1 cells at 8, 32, and 64-day time course of BMP treatment after GF withdrawal (GF). Error bars denote SD of four independent experiments. c Frequency of MKP1 promoter methylation after BMP4 continous treatment at the indicated time points. d mRNA expression of MKP1 was analyzed in U87 and GNS166 lines under increasing 5-AZA concentrations. e Analysis of MKP1 mRNA expression levels in U251 cells with knockdown of SOX2 ( shSOX2 ) or SOX9 ( shSOX9 ) related to the control ( pLKO ) condition ( n = 3). qRT-PCR data are normalized to GAPDH expression. f MKP1 levels in U87 cells with SOX2 or SOX9 overexpression compared to control cells ( n = 3). g Association analysis of MKP1 with SOX2 and SOX9 in the human glioblastoma TCGA cohort from R2: Genomics Analysis and Visualization Platform http://r2.amc.nl . The analysis showed a negative correlation between MKP1 and SOX2 ( R −0.207, p value 1.3e−06), and MKP1 and SOX9 ( R −0.169, p value 1.7e−04)
Article Snippet: For SOX2 or SOX9 knockdown, cells were infected with
Techniques: Expressing, RNA Sequencing, Methylation, Knockdown, Control, Quantitative RT-PCR, Over Expression
Journal: Developmental cell
Article Title: A Yap-Myc-Sox2-p53 Regulatory Network Dictates Metabolic Homeostasis and Differentiation in Kras-driven Pancreatic Ductal Adenocarcinomas
doi: 10.1016/j.devcel.2019.07.022
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: Lenti- or retro-viral plasmids pLKO.1 shSox2 HM a (#26353),
Techniques: Recombinant, Viability Assay, Imaging, TUNEL Assay, Methylation, Plasmid Preparation, Polymer, cDNA Synthesis, SYBR Green Assay, Bicinchoninic Acid Protein Assay, DNA Extraction, Amplification, Knock-Out, CRISPR, Software